Genotyping and primer details


Genotyping and primer details for 121F08 - At5g10350
Line ID 121F08
Locus At5g10350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3256981 of line 121F08 Chr5:3256981
   
Insertion allele amplicon 8474 - 19ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19ra: 5'-AGAGGAGATTAAAGGAGATGGAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3257529
   
wt amplicon (over InsPos) 20rt - 19ra
wt amplicon start Chr5:3256601
wt amplicon end Chr5:3257529
wt amplicon size 929 bp
Locus specific primer 19ra: 5'-AGAGGAGATTAAAGGAGATGGAGG-3'
2nd locus specific primer 20rt: 5'-ACCATCTCCTCAAGGATAACAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11