Genotyping and primer details


Genotyping and primer details for 122H04 - At5g37475
Line ID 122H04
Locus At5g37475 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:14866303 of line 122H04 Chr5:14866303
   
Insertion allele amplicon 8474 - 10uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10uv: 5'-ATCACTTTACGCCAAAGACTTGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:14865912
   
wt amplicon (over InsPos) 53vd - 10uv
wt amplicon start Chr5:14865912
wt amplicon end Chr5:14866737
wt amplicon size 826 bp
Locus specific primer 10uv: 5'-ATCACTTTACGCCAAAGACTTGAT-3'
2nd locus specific primer 53vd: 5'-GCAGAAAAGCTTTCACTATATCGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11