Genotyping and primer details


Genotyping and primer details for 125D10 - At2g24740
Line ID 125D10
Locus At2g24740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10531298 of line 125D10 Chr2:10531298
   
Insertion allele amplicon 3144 - IN38
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer IN38: 5'-ACTAGTCCAATAGACCGAGAAGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10531603
   
wt amplicon (over InsPos) 43rd - IN38
wt amplicon start Chr2:10530901
wt amplicon end Chr2:10531603
wt amplicon size 703 bp
Locus specific primer IN38: 5'-ACTAGTCCAATAGACCGAGAAGCA-3'
2nd locus specific primer 43rd: 5'-GACCATCCACTCCACTCTCTTTAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11