Genotyping and primer details


Genotyping and primer details for 126F10 - At5g45400
Line ID 126F10
Locus At5g45400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18401299 of line 126F10 Chr5:18401299
   
Insertion allele amplicon 8474 - 21ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21ra: 5'-ATTTCTGCTATACGGCTTGTCCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18400869
   
wt amplicon (over InsPos) 18rt - 21ra
wt amplicon start Chr5:18400869
wt amplicon end Chr5:18401689
wt amplicon size 821 bp
Locus specific primer 21ra: 5'-ATTTCTGCTATACGGCTTGTCCTA-3'
2nd locus specific primer 18rt: 5'-CCATTGTTGATGATAATGATGAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11