Genotyping and primer details


Genotyping and primer details for 128D08 - At2g34160
Line ID 128D08
Locus At2g34160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14426816 of line 128D08 Chr2:14426816
   
Insertion allele amplicon 8474 - KH26
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KH26: 5'-ATTTATGGCCGAGATGTTTTATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14427387
   
wt amplicon (over InsPos) 20ep - KH26
wt amplicon start Chr2:14426651
wt amplicon end Chr2:14427387
wt amplicon size 737 bp
Locus specific primer KH26: 5'-ATTTATGGCCGAGATGTTTTATTG-3'
2nd locus specific primer 20ep: 5'-TTGTTGTTTGGATTTTGTTCAGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11