Genotyping and primer details


Genotyping and primer details for 130H01 - At1g21610
Line ID 130H01
Locus At1g21610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7577476 of line 130H01 Chr1:7577476
   
Insertion allele amplicon 8474 - 08dl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08dl: 5'-TAAAGTTGCTAGGATTGCGGTACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7576846
   
wt amplicon (over InsPos) 09ec - 08dl
wt amplicon start Chr1:7576846
wt amplicon end Chr1:7578013
wt amplicon size 1168 bp
Locus specific primer 08dl: 5'-TAAAGTTGCTAGGATTGCGGTACT-3'
2nd locus specific primer 09ec: 5'-TCTAACAAACCACCTTGAGCTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11