Genotyping and primer details


Genotyping and primer details for 131C12 - At1g60590
Line ID 131C12
Locus At1g60590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22316132 of line 131C12 Chr1:22316132
   
Insertion allele amplicon 8474 - 05ve
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05ve: 5'-TTGAGGACTCTCAACAATTCAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22316518
   
wt amplicon (over InsPos) 81wa - 05ve
wt amplicon start Chr1:22315539
wt amplicon end Chr1:22316518
wt amplicon size 980 bp
Locus specific primer 05ve: 5'-TTGAGGACTCTCAACAATTCAATG-3'
2nd locus specific primer 81wa: 5'-CAAAAATACAAAAACAGAGTTTGGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11