Genotyping and primer details


Genotyping and primer details for 131G09 - At4g33400
Line ID 131G09
Locus At4g33400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16079511 of line 131G09 Chr4:16079511
   
Insertion allele amplicon 3144 - 01gj
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 01gj: 5'-GTCTAATTTTGCCATCAAGAGAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16079042
   
wt amplicon (over InsPos) 02hn - 01gj
wt amplicon start Chr4:16079042
wt amplicon end Chr4:16079967
wt amplicon size 926 bp
Locus specific primer 01gj: 5'-GTCTAATTTTGCCATCAAGAGAGC-3'
2nd locus specific primer 02hn: 5'-CCGTGAAAAGGTATCAGATGAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11