Genotyping and primer details


Genotyping and primer details for 132F04 - At3g47090
Line ID 132F04
Locus At3g47090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17343380 of line 132F04 Chr3:17343380
   
Insertion allele amplicon 8474 - 62wr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 62wr: 5'-TGGGATATAATCATCTGGAAGGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17344068
   
Insertion allele amplicon 3144 - NR74
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer NR74: 5'-GGCTTTAGTTTCAGTTCCTTGATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17342775
   
wt amplicon (over InsPos) PB41 - NR74
wt amplicon start Chr3:17342775
wt amplicon end Chr3:17343779
wt amplicon size 1005 bp
Locus specific primer NR74: 5'-GGCTTTAGTTTCAGTTCCTTGATG-3'
2nd locus specific primer PB41: 5'-AATTGGTAAAAACAGGATGACAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11