Genotyping and primer details


Genotyping and primer details for 134G02 - At3g01150
Line ID 134G02
Locus At3g01150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:52699 of line 134G02 Chr3:52699
   
Insertion allele amplicon 8474 - 09vf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09vf: 5'-TGATTTTACGATTTCGAGTGTGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:52380
   
wt amplicon (over InsPos) 22vu - 09vf
wt amplicon start Chr3:52380
wt amplicon end Chr3:53293
wt amplicon size 914 bp
Locus specific primer 09vf: 5'-TGATTTTACGATTTCGAGTGTGAG-3'
2nd locus specific primer 22vu: 5'-ACACCAACGAATCAAACATATGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11