Genotyping and primer details


Genotyping and primer details for 135C12 - At2g22360
Line ID 135C12
Locus At2g22360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9500062 of line 135C12 Chr2:9500062
   
Insertion allele amplicon 8474 - 11kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11kh: 5'-TTTTTCTCCTCTTTGCTCAATCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9500398
   
wt amplicon (over InsPos) 02ky - 11kh
wt amplicon start Chr2:9499522
wt amplicon end Chr2:9500398
wt amplicon size 877 bp
Locus specific primer 11kh: 5'-TTTTTCTCCTCTTTGCTCAATCTC-3'
2nd locus specific primer 02ky: 5'-GTTCAAAACACAGATGCACAGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11