Genotyping and primer details


Genotyping and primer details for 137A04 - At5g09630
Line ID 137A04
Locus At5g09630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2987507 of line 137A04 Chr5:2987507
   
Insertion allele amplicon 8474 - 02gj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02gj: 5'-AAGAAGTCAAGGACTCCACTACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2988135
   
wt amplicon (over InsPos) 07hs - 02gj
wt amplicon start Chr5:2987135
wt amplicon end Chr5:2988135
wt amplicon size 1001 bp
Locus specific primer 02gj: 5'-AAGAAGTCAAGGACTCCACTACCA-3'
2nd locus specific primer 07hs: 5'-ATCAAATGCATCTCTCACAGTCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11