Genotyping and primer details


Genotyping and primer details for 137D11 - At5g42770
Line ID 137D11
Locus At5g42770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17152424 of line 137D11 Chr5:17152424
   
Insertion allele amplicon 8474 - 10vf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10vf: 5'-CGATATTCAAGTTCAGCAATCTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17152111
   
wt amplicon (over InsPos) 21vu - 10vf
wt amplicon start Chr5:17152111
wt amplicon end Chr5:17153261
wt amplicon size 1151 bp
Locus specific primer 10vf: 5'-CGATATTCAAGTTCAGCAATCTCA-3'
2nd locus specific primer 21vu: 5'-CTCTATGTTTTCACCATCAGGGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11