Genotyping and primer details


Genotyping and primer details for 138C12 - At1g52190
Line ID 138C12
Locus At1g52190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19436393 of line 138C12 Chr1:19436393
   
Insertion allele amplicon 8474 - 11de
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11de: 5'-GTGGTAACATTGCAGTTAGCCATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19436843
   
wt amplicon (over InsPos) 15dy - 11de
wt amplicon start Chr1:19435785
wt amplicon end Chr1:19436843
wt amplicon size 1059 bp
Locus specific primer 11de: 5'-GTGGTAACATTGCAGTTAGCCATA-3'
2nd locus specific primer 15dy: 5'-AGATTAATTTCATAGCGTGCCAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11