Genotyping and primer details


Genotyping and primer details for 139C01 - At5g01530
Line ID 139C01
Locus At5g01530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:209259 of line 139C01 Chr5:209259
   
Insertion allele amplicon 3144 - DE36
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer DE36: 5'-TGCTAACGCAAACTGTAAGAAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:209784
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11