Genotyping and primer details


Genotyping and primer details for 140G07 - At4g18440
Line ID 140G07
Locus At4g18440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10187941 of line 140G07 Chr4:10187941
   
Insertion allele amplicon 8474 - 23ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23ra: 5'-GAATTGGCGATAAATTGATATGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10188587
   
wt amplicon (over InsPos) 05se - 23ra
wt amplicon start Chr4:10187449
wt amplicon end Chr4:10188587
wt amplicon size 1139 bp
Locus specific primer 23ra: 5'-GAATTGGCGATAAATTGATATGCT-3'
2nd locus specific primer 05se: 5'-GAGTCACGTATGGGTTGAATGTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11