Genotyping and primer details


Genotyping and primer details for 141B10 - At1g55110
Line ID 141B10
Locus At1g55110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20562034 of line 141B10 Chr1:20562034
   
Insertion allele amplicon 8474 - 12de
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12de: 5'-GAGATCAAGCAAGTGTGTCTTCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20562540
   
wt amplicon (over InsPos) 17dy - 12de
wt amplicon start Chr1:20561709
wt amplicon end Chr1:20562540
wt amplicon size 832 bp
Locus specific primer 12de: 5'-GAGATCAAGCAAGTGTGTCTTCAG-3'
2nd locus specific primer 17dy: 5'-GCATTTGTATTCTTTAGTGCCACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11