Genotyping and primer details


Genotyping and primer details for 141D01 - At1g15930
Line ID 141D01
Locus At1g15930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5471732 of line 141D01 Chr1:5471732
   
Insertion allele amplicon 8474 - 09cv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09cv: 5'-TAGGAGGATAAACTGAGCGACAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5472469
   
wt amplicon (over InsPos) 18dy - 09cv
wt amplicon start Chr1:5471414
wt amplicon end Chr1:5472469
wt amplicon size 1056 bp
Locus specific primer 09cv: 5'-TAGGAGGATAAACTGAGCGACAAT-3'
2nd locus specific primer 18dy: 5'-TCCAAATTTCATTCGTATCCTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11