Genotyping and primer details


Genotyping and primer details for 141F04 - At1g76185
Line ID 141F04
Locus At1g76185 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28590654 of line 141F04 Chr1:28590654
   
Insertion allele amplicon 8474 - 11op
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11op: 5'-TGCTTTGTGATTTTCATACAGAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28591102
   
wt amplicon (over InsPos) 19tf - 11op
wt amplicon start Chr1:28590178
wt amplicon end Chr1:28591102
wt amplicon size 925 bp
Locus specific primer 11op: 5'-TGCTTTGTGATTTTCATACAGAGG-3'
2nd locus specific primer 19tf: 5'-AAAGAGTAACGGTAAAAGCCCAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11