Genotyping and primer details


Genotyping and primer details for 142B12 - At2g01250
Line ID 142B12
Locus At2g01250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:133590 of line 142B12 Chr2:133590
   
Insertion allele amplicon 3144 - BK86
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer BK86: 5'-GGTTAAGCTTTCCGTAACCTCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:133299
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11