Genotyping and primer details


Genotyping and primer details for 142D03 - At1g22230
Line ID 142D03
Locus At1g22230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7851069 of line 142D03 Chr1:7851069
   
Insertion allele amplicon 8474 - 08us
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08us: 5'-ATCAGTTGATTCTGTGTCAACGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7851322
   
wt amplicon (over InsPos) 48vc - 08us
wt amplicon start Chr1:7850598
wt amplicon end Chr1:7851322
wt amplicon size 725 bp
Locus specific primer 08us: 5'-ATCAGTTGATTCTGTGTCAACGTC-3'
2nd locus specific primer 48vc: 5'-AAGATGAAGATGAAAGTTCAGGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11