Genotyping and primer details


Genotyping and primer details for 142F02 - At2g22660
Line ID 142F02
Locus At2g22660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9628013 of line 142F02 Chr2:9628013
   
Insertion allele amplicon 8474 - MO03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MO03: 5'-AAAAATCGGGTTTTAAGATTCTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9627564
   
Insertion allele amplicon 8474 - MO09
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MO09: 5'-AAAAATCGGGTTTTAAGATTCTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9627564
   
wt amplicon (over InsPos) 19dy - MO09
wt amplicon start Chr2:9627564
wt amplicon end Chr2:9628526
wt amplicon size 963 bp
Locus specific primer MO09: 5'-AAAAATCGGGTTTTAAGATTCTCC-3'
2nd locus specific primer 19dy: 5'-AACACACAGAAACAGAATCAGGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11