Genotyping and primer details


Genotyping and primer details for 143C11 - At1g54790
Line ID 143C11
Locus At1g54790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20442976 of line 143C11 Chr1:20442976
   
Insertion allele amplicon 8474 - 09qz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09qz: 5'-AATCCCCAAAACATTAACATGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20443356
   
wt amplicon (over InsPos) 07rt - 09qz
wt amplicon start Chr1:20442617
wt amplicon end Chr1:20443356
wt amplicon size 740 bp
Locus specific primer 09qz: 5'-AATCCCCAAAACATTAACATGAAA-3'
2nd locus specific primer 07rt: 5'-GTTTACAATTGGAGCCATTGAGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11