Genotyping and primer details


Genotyping and primer details for 144G10 - At3g17770
Line ID 144G10
Locus At3g17770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6085664 of line 144G10 Chr3:6085664
   
Insertion allele amplicon 8474 - BK88
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BK88: 5'-GTTTTGATAGCGGCTTAATACTCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6085986
   
wt amplicon (over InsPos) AP38 - BK88
wt amplicon start Chr3:6085295
wt amplicon end Chr3:6085986
wt amplicon size 692 bp
Locus specific primer BK88: 5'-GTTTTGATAGCGGCTTAATACTCG-3'
2nd locus specific primer AP38: 5'-CAGAACTCTACACCAACCACGTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11