Genotyping and primer details


Genotyping and primer details for 144H03 - At4g30130
Line ID 144H03
Locus At4g30130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14736313 of line 144H03 Chr4:14736313
   
Insertion allele amplicon 8474 - 03um
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03um: 5'-AGCAGATAGTTTCTTTGGGATGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14735799
   
wt amplicon (over InsPos) 27vl - 03um
wt amplicon start Chr4:14735799
wt amplicon end Chr4:14736499
wt amplicon size 701 bp
Locus specific primer 03um: 5'-AGCAGATAGTTTCTTTGGGATGAA-3'
2nd locus specific primer 27vl: 5'-AAGAAGTCTCAGAACCACATTCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11