Genotyping and primer details


Genotyping and primer details for 147B11 - At4g21900
Line ID 147B11
Locus At4g21900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:11619332 of line 147B11 Chr4:11619332
   
Insertion allele amplicon 8474 - BM02
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BM02: 5'-GATGAAACCCTTACCCTGACATTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:11618831
   
wt amplicon (over InsPos) 01tf - BM02
wt amplicon start Chr4:11618831
wt amplicon end Chr4:11619768
wt amplicon size 938 bp
Locus specific primer BM02: 5'-GATGAAACCCTTACCCTGACATTA-3'
2nd locus specific primer 01tf: 5'-AAAGTTTTCGTCCTTTGTGTGACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11