Genotyping and primer details


Genotyping and primer details for 150D10 - At5g65260
Line ID 150D10
Locus At5g65260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26082058 of line 150D10 Chr5:26082058
   
Insertion allele amplicon 8474 - 25ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25ra: 5'-AATACTCCACTCAAACATTGTCCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26081471
   
wt amplicon (over InsPos) 17rt - 25ra
wt amplicon start Chr5:26081471
wt amplicon end Chr5:26082379
wt amplicon size 909 bp
Locus specific primer 25ra: 5'-AATACTCCACTCAAACATTGTCCC-3'
2nd locus specific primer 17rt: 5'-CAGTTTTTGTTGGCAATGTAAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11