Genotyping and primer details


Genotyping and primer details for 151C09 - At2g27190
Line ID 151C09
Locus At2g27190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11622216 of line 151C09 Chr2:11622216
   
Insertion allele amplicon 8474 - ML67
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer ML67: 5'-CAGGGGATCTAGGACAAACTTATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11622682
   
Insertion allele amplicon 8474 - ML68
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer ML68: 5'-CTGTTTTTAATCTCTAGCAACCCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11621521
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11