Genotyping and primer details


Genotyping and primer details for 153C03 - At2g41790
Line ID 153C03
Locus At2g41790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17433087 of line 153C03 Chr2:17433087
   
Insertion allele amplicon 8474 - 36ci
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 36ci: 5'-CTCTCAGCTATTTGTGAGACGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17433380
   
Insertion allele amplicon 8474 - CP32
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CP32: 5'-GTAAACTGCAGAAAAGCTAATGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17432689
   
wt amplicon (over InsPos) CP32 - 36ci
wt amplicon start Chr2:17432689
wt amplicon end Chr2:17433380
wt amplicon size 692 bp
Locus specific primer 36ci: 5'-CTCTCAGCTATTTGTGAGACGAAA-3'
2nd locus specific primer CP32: 5'-GTAAACTGCAGAAAAGCTAATGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11