Genotyping and primer details


Genotyping and primer details for 159B12 - At2g42950
Line ID 159B12
Locus At2g42950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17864520 of line 159B12 Chr2:17864520
   
Insertion allele amplicon 8474 - 10cv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10cv: 5'-ATGGAGATTCGTCTCTCAGAATGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17864171
   
wt amplicon (over InsPos) 21dy - 10cv
wt amplicon start Chr2:17864171
wt amplicon end Chr2:17865051
wt amplicon size 881 bp
Locus specific primer 10cv: 5'-ATGGAGATTCGTCTCTCAGAATGT-3'
2nd locus specific primer 21dy: 5'-AGCAATAAGCATTTCCTGGTAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11