Genotyping and primer details


Genotyping and primer details for 160E03 - At3g28590
Line ID 160E03
Locus At3g28590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:10718507 of line 160E03 Chr3:10718507
   
Insertion allele amplicon 8474 - ML56
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer ML56: 5'-TGCTGCTAGATTTCTGAAAATTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:10718802
   
wt amplicon (over InsPos) 18sq - ML56
wt amplicon start Chr3:10717643
wt amplicon end Chr3:10718802
wt amplicon size 1160 bp
Locus specific primer ML56: 5'-TGCTGCTAGATTTCTGAAAATTGA-3'
2nd locus specific primer 18sq: 5'-CCATTTAGAACCCATTTGTCATGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11