Genotyping and primer details


Genotyping and primer details for 167D03 - At4g40050
Line ID 167D03
Locus At4g40050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:18563303 of line 167D03 Chr4:18563303
   
Insertion allele amplicon 8474 - 13vf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13vf: 5'-TTCCAGAAAAACAAACAAACAGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:18562924
   
wt amplicon (over InsPos) 78wb - 13vf
wt amplicon start Chr4:18562924
wt amplicon end Chr4:18563952
wt amplicon size 1029 bp
Locus specific primer 13vf: 5'-TTCCAGAAAAACAAACAAACAGTG-3'
2nd locus specific primer 78wb: 5'-GAAAGGTGATCATCAACAAAACAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11