Genotyping and primer details


Genotyping and primer details for 169D09 - At3g56740
Line ID 169D09
Locus At3g56740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21016204 of line 169D09 Chr3:21016204
   
Insertion allele amplicon 8474 - 03ht
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03ht: 5'-CCTGAGAACAAGATAAAAACCTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21016642
   
wt amplicon (over InsPos) 06ix - 03ht
wt amplicon start Chr3:21015850
wt amplicon end Chr3:21016642
wt amplicon size 793 bp
Locus specific primer 03ht: 5'-CCTGAGAACAAGATAAAAACCTGC-3'
2nd locus specific primer 06ix: 5'-GGTATTTCGCATTTGGTGTAGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11