Genotyping and primer details


Genotyping and primer details for 169D12 - At2g30942
Line ID 169D12
Locus At2g30942 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13171337 of line 169D12 Chr2:13171337
   
Insertion allele amplicon 8474 - BL46
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BL46: 5'-GAAAGATGCCTGTGGAATAAGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13171831
   
wt amplicon (over InsPos) 07tx - BL46
wt amplicon start Chr2:13171017
wt amplicon end Chr2:13171831
wt amplicon size 815 bp
Locus specific primer BL46: 5'-GAAAGATGCCTGTGGAATAAGAAG-3'
2nd locus specific primer 07tx: 5'-GGTGGGAACGATACCTATTCAGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11