Genotyping and primer details


Genotyping and primer details for 170D03 - At1g34210
Line ID 170D03
Locus At1g34210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:12460023 of line 170D03 Chr1:12460023
   
Insertion allele amplicon 8474 - 31wp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 31wp: 5'-AATGGTCAACTGCTGATAATTTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:12460953
   
Insertion allele amplicon 8474 - MN61
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MN61: 5'-CTAGAGAATCTGGAATTGGACCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:12460240
   
Insertion allele amplicon 8474 - NH61
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NH61: 5'-CACTGCACAGTTTGAGAGCTAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:12459589
   
wt amplicon (over InsPos) MN61 - NH61
wt amplicon start Chr1:12459589
wt amplicon end Chr1:12460240
wt amplicon size 652 bp
Locus specific primer NH61: 5'-CACTGCACAGTTTGAGAGCTAATC-3'
2nd locus specific primer MN61: 5'-CTAGAGAATCTGGAATTGGACCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11