Genotyping and primer details


Genotyping and primer details for 171F04 - At5g24240
Line ID 171F04
Locus At5g24240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:8231462 of line 171F04 Chr5:8231462
   
Insertion allele amplicon 8474 - 04bq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04bq: 5'-AAACTCATCTTCGGTTGTCTCTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:8231167
   
wt amplicon (over InsPos) 16dk - 04bq
wt amplicon start Chr5:8231167
wt amplicon end Chr5:8231959
wt amplicon size 793 bp
Locus specific primer 04bq: 5'-AAACTCATCTTCGGTTGTCTCTGT-3'
2nd locus specific primer 16dk: 5'-GAAGTAGCAGCCTACATTTTGGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11