Genotyping and primer details


Genotyping and primer details for 173D05 - At5g02960
Line ID 173D05
Locus At5g02960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:693220 of line 173D05 Chr5:693220
   
Insertion allele amplicon 8474 - 72lx
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 72lx: 5'-TCTCTTGGGTTTTGAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:694050
   
wt amplicon (over InsPos) 47vc - 72lx
wt amplicon start Chr5:692688
wt amplicon end Chr5:694050
wt amplicon size 1363 bp
Locus specific primer 72lx: 5'-TCTCTTGGGTTTTGAAGC-3'
2nd locus specific primer 47vc: 5'-AGACTAAGGTCACGATGAAGGGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11