Genotyping and primer details


Genotyping and primer details for 174C12 - At1g02890
Line ID 174C12
Locus At1g02890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:648496 of line 174C12 Chr1:648496
   
Insertion allele amplicon 8474 - MZ84
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MZ84: 5'-CCAAGGAAGCAGATACTACCAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:649129
   
wt amplicon (over InsPos) 16hf - MZ84
wt amplicon start Chr1:648186
wt amplicon end Chr1:649129
wt amplicon size 944 bp
Locus specific primer MZ84: 5'-CCAAGGAAGCAGATACTACCAAAG-3'
2nd locus specific primer 16hf: 5'-TATATACACATCAGTGTTCCCCGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11