Genotyping and primer details


Genotyping and primer details for 176D01 - At1g70060
Line ID 176D01
Locus At1g70060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26385252 of line 176D01 Chr1:26385252
   
Insertion allele amplicon 8474 - 17bk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17bk: 5'-GAAGTCTCCGATAACTAGGTGTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26385858
   
wt amplicon (over InsPos) 06cm - 17bk
wt amplicon start Chr1:26384926
wt amplicon end Chr1:26385858
wt amplicon size 933 bp
Locus specific primer 17bk: 5'-GAAGTCTCCGATAACTAGGTGTGC-3'
2nd locus specific primer 06cm: 5'-TACGTAAGGATGATGATTCTGCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11