Genotyping and primer details


Genotyping and primer details for 176F04 - At1g13160
Line ID 176F04
Locus At1g13160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4484691 of line 176F04 Chr1:4484691
   
Insertion allele amplicon 8474 - CM46
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CM46: 5'-CTCATCTTACGAATGGTTTGAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4485280
   
wt amplicon (over InsPos) 01mj - CM46
wt amplicon start Chr1:4484252
wt amplicon end Chr1:4485280
wt amplicon size 1029 bp
Locus specific primer CM46: 5'-CTCATCTTACGAATGGTTTGAACA-3'
2nd locus specific primer 01mj: 5'-AAAACTTGTAGCACTCTGCAAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11