Genotyping and primer details


Genotyping and primer details for 178G01 - At1g17030
Line ID 178G01
Locus At1g17030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5824292 of line 178G01 Chr1:5824292
   
Insertion allele amplicon 8474 - 10vh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10vh: 5'-CATACTTTTCCAAACGGGACTAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5823908
   
wt amplicon (over InsPos) 27vu - 10vh
wt amplicon start Chr1:5823908
wt amplicon end Chr1:5824972
wt amplicon size 1065 bp
Locus specific primer 10vh: 5'-CATACTTTTCCAAACGGGACTAAA-3'
2nd locus specific primer 27vu: 5'-AAACACAATCGAGAGCTTAAAGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11