Genotyping and primer details


Genotyping and primer details for 179D07 - At5g13020
Line ID 179D07
Locus At5g13020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:4131830 of line 179D07 Chr5:4131830
   
Insertion allele amplicon 8474 - 02en
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02en: 5'-AGCTGAGGAACACTAGACTGAAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4131391
   
Insertion allele amplicon 8474 - 03ff
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03ff: 5'-AGGAACACTAGACTGAAGGCTGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:4131396
   
wt amplicon (over InsPos) 07fz - 02en
wt amplicon start Chr5:4131391
wt amplicon end Chr5:4132356
wt amplicon size 966 bp
Locus specific primer 02en: 5'-AGCTGAGGAACACTAGACTGAAGG-3'
2nd locus specific primer 07fz: 5'-GAAGGCAACATGTTTGTCTGTTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11