Genotyping and primer details


Genotyping and primer details for 179G02 - At4g26630
Line ID 179G02
Locus At4g26630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13432167 of line 179G02 Chr4:13432167
   
Insertion allele amplicon 8474 - 19ew
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19ew: 5'-AAAACAGGATAATGACCAAACCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13431648
   
wt amplicon (over InsPos) 02fq - 19ew
wt amplicon start Chr4:13431648
wt amplicon end Chr4:13432687
wt amplicon size 1040 bp
Locus specific primer 19ew: 5'-AAAACAGGATAATGACCAAACCTG-3'
2nd locus specific primer 02fq: 5'-CCATTTCTGGTTGTCCAAGTTTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11