Genotyping and primer details


Genotyping and primer details for 179G08 - At1g74440
Line ID 179G08
Locus At1g74440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27979841 of line 179G08 Chr1:27979841
   
Insertion allele amplicon 8474 - 11qz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11qz: 5'-CACTCTCAATTAGGGTTCCTCAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27980212
   
wt amplicon (over InsPos) 04rp - 11qz
wt amplicon start Chr1:27979428
wt amplicon end Chr1:27980212
wt amplicon size 785 bp
Locus specific primer 11qz: 5'-CACTCTCAATTAGGGTTCCTCAAG-3'
2nd locus specific primer 04rp: 5'-AAAGAAAGGACCCATAAGAAAAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11