Genotyping and primer details


Genotyping and primer details for 180C03 - At1g16470
Line ID 180C03
Locus At1g16470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5623772 of line 180C03 Chr1:5623772
   
Insertion allele amplicon 8474 - 12hw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12hw: 5'-TCTCAAATTGCAAGTGAAAAGCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5624102
   
wt amplicon (over InsPos) 21ix - 12hw
wt amplicon start Chr1:5623204
wt amplicon end Chr1:5624102
wt amplicon size 899 bp
Locus specific primer 12hw: 5'-TCTCAAATTGCAAGTGAAAAGCTA-3'
2nd locus specific primer 21ix: 5'-TTGCTGCGACTAATTTGTTGTACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11