Genotyping and primer details


Genotyping and primer details for 182H04 - At2g43840
Line ID 182H04
Locus At2g43840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18157867 of line 182H04 Chr2:18157867
   
Insertion allele amplicon 8474 - JK52
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JK52: 5'-TAGGAGTGACGAAAGTAGGCAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18158200
   
wt amplicon (over InsPos) 07bd - JK52
wt amplicon start Chr2:18157384
wt amplicon end Chr2:18158200
wt amplicon size 817 bp
Locus specific primer JK52: 5'-TAGGAGTGACGAAAGTAGGCAAAT-3'
2nd locus specific primer 07bd: 5'-TTCTGACTTTGTTATTACGTCCCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11