Genotyping and primer details


Genotyping and primer details for 182H05 - At3g47110
Line ID 182H05
Locus At3g47110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17348162 of line 182H05 Chr3:17348162
   
Insertion allele amplicon 3144 - MN52
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer MN52: 5'-AGTATGAAGATAAACCAGAGCCGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17347759
   
Insertion allele amplicon 8474 - NH56
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NH56: 5'-CCATTCCAGATATAAGAGGGTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17348618
   
wt amplicon (over InsPos) NH56 - MN52
wt amplicon start Chr3:17347759
wt amplicon end Chr3:17348618
wt amplicon size 860 bp
Locus specific primer MN52: 5'-AGTATGAAGATAAACCAGAGCCGA-3'
2nd locus specific primer NH56: 5'-CCATTCCAGATATAAGAGGGTTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11