Genotyping and primer details


Genotyping and primer details for 183C05 - At5g17390
Line ID 183C05
Locus At5g17390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5728068 of line 183C05 Chr5:5728068
   
Insertion allele amplicon 8474 - 13uw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13uw: 5'-TGAAGAAAACAGAAGAGCTTGACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5727458
   
wt amplicon (over InsPos) 51wd - 13uw
wt amplicon start Chr5:5727458
wt amplicon end Chr5:5728662
wt amplicon size 1205 bp
Locus specific primer 13uw: 5'-TGAAGAAAACAGAAGAGCTTGACA-3'
2nd locus specific primer 51wd: 5'-GGCGTTTGGTGGTGATTAAGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11