Genotyping and primer details


Genotyping and primer details for 183C07 - At1g66480
Line ID 183C07
Locus At1g66480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24806285 of line 183C07 Chr1:24806285
   
Insertion allele amplicon 8474 - AJ21
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AJ21: 5'-CGATAAATCATTTGATGATCGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24805936
   
Insertion allele amplicon 8474 - KA28
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KA28: 5'-CTAGAACCATGTTTTTGAGAAGCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24806603
   
wt amplicon (over InsPos) AJ21 - KA28
wt amplicon start Chr1:24805936
wt amplicon end Chr1:24806603
wt amplicon size 668 bp
Locus specific primer KA28: 5'-CTAGAACCATGTTTTTGAGAAGCC-3'
2nd locus specific primer AJ21: 5'-CGATAAATCATTTGATGATCGAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11