Genotyping and primer details


Genotyping and primer details for 183F09 - At1g29760
Line ID 183F09
Locus At1g29760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:10423314 of line 183F09 Chr1:10423314
   
Insertion allele amplicon 8474 - BL67
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BL67: 5'-CAATCGAATTTCAAGTCAGTTTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10422934
   
wt amplicon (over InsPos) 07ua - BL67
wt amplicon start Chr1:10422934
wt amplicon end Chr1:10423569
wt amplicon size 636 bp
Locus specific primer BL67: 5'-CAATCGAATTTCAAGTCAGTTTCA-3'
2nd locus specific primer 07ua: 5'-CACATGAGAAACTATTTCAAGCCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11